Advanced semen analysis

Advanced semen analysis : One of the main reasons for infertility in couples - in up to 50% of cases - is male infertility. Male infertility is not necessarily related to male health, as a healthy man can produce poor quality sperm that make conception difficult.

Advanced Semen Analysis in Bangalore

It is therefore very important to perform a semen analysis to determine the fertility potential of the man. A normal semen analysis or seminogram looks at the concentration, motility and morphology of the sperm cells in a semen sample.

While these factors are important in assessing basic sperm quality, there are other components that are far more important in determining the capacity of the sperm to create pregnancy. These other factors can give patients a better insight into the diagnosis and thus a more appropriate treatment plan. These evaluations are categorized as Advanced Semen Analysis.

In the Joshi's Andrology & Urology Centre laboratory, it is possible to perform an advanced Semen analysis.

Advanced semen analysis: DNA fragmentation index test for sperm.

The presence of fragmented DNA in the nucleus of sperm cells has a negative impact on reproductive results. The sperm DNA fragmentation test measures the stability of the chromatin and estimate the percentage of sperm cells that have fragmented DNA.

Any couple doing an assisted reproductive treatment can take the DNA fragmentation test for sperm. This test is especially recommended for men over 35 years of age, history of recurrent miscarriages, history of failed IVF and IUI, history of exposure to gonadotoxins, who have a genitourinary infection or who have received cancer treatment etc..

The DNA fragmentation test can be useful for couples who have had multiple miscarriages, unsuccessfully completed multiple cycles, or who cannot find a reason for their infertility.

·         Advanced semen analysis: Apoptosis -Annexin V test

Apoptosis or cell death plays an important role in spermatogenesis by eliminating sperm cells with structural or functional defects. The presence of an abnormally high level of dead sperm in the ejaculate can adversely affect male fertility.

The Apoptosis -Annexin V test determines the number of damaged sperm cells in the ejaculate and / or the sperm samples reserved for assisted reproduction. The assay uses the Annexin V protein to mark the damaged cells, which are then counted. The quality of samples with abnormally high apoptosis can be improved by specifically removing damaged sperm cells, using the Annexin V MACS Technique.

·         Advanced semen analysis: Oxidative Stress test (ROS)

The sperm cells produce small amounts of reactive oxygen species (ROS, short for reactive oxygen species) that are essential for their normal functioning. This amount of ROS is controlled by the antioxidants present in the fluid from the prostate and seminal vesicles.

However, as the balance between reactive oxygen component production and antioxidant activity shifts, ROS levels will increase. This causes oxidative stress, which damages the sperm and decreases fertility. Increasing the levels of ROS can be reduced through lifestyle changes and a diet rich in antioxidants.

 

·         Advanced Sperm Analysis: Y Chromosome Microdeletion Test

The microdeletions in the AZF factor of the Y chromosome are responsible for about 10-15% of azoospermia and 5-10% of oligospermia cases in men. The Y chromosome microdeletion test examines parts of the Y chromosome by PCR: AZFa, AZFb, AZFc. This identifies possible causes of male infertility.

 

 

Joshi's Andrology & Urology Centre

Address: Neelamma Complex, Opposite Unlimited mall, No 44/2 1st floor, 60 Feet Rd, G Block, Sahakar Nagar, Bengaluru, Karnataka 560092

Appointments: https://joshiuroandrology.com/Pages/Contact-Us, 

Phone: 080 4207 0577